Description 1. In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMaxi3Multi. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. Curcumin binding assay. Biochrom New EX Read 400 Microplate Reader. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. However, no piece of equipment is immune to breaking down. SpectraMax M5. The SoftMax® Touch Software is installed in. Figure 3. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Read height should be optimized for . Find regional support. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. The calculated LOQ values were 0. i3. Published Dec 16, 2020 | Updated Dec 16, 2020. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. 99 to $1999. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Each heating element is independently. Unplug the power cable from the back of the instrument. It allows you to explore cellular pathways and protein activation and expression in one system. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Monday to Friday 8am - 5pm GMT. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. The Results. Viewing this answer requires an authorized account. 2 nM was calculated using SoftMax Pro Software. 3. gen) on a Spectramax i3X luminometer. . which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. ü; ü. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. . 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Reaction catalyzed by the firefly luciferase. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. SoftMax Pro. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. . Antigen Quantitation Protocol. Instrument. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. Overview of Western Blot Cartridge. FlexStation® 3 Multi-Mode Microplate Reader. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. Introduction. Tristar 5 Multimode Microplate Reader. 0. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. View the Molecular Devices website. 5. SpectraMax i3x, onboard luminescence 121. , Fisher Scienti fi c, cat. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Winnersh Triangle. ImageXpress Confocal HT. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. . Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Shaking and incubations were performed using an on-deck orbital shaker. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The protocols are also available on the SoftMax Pro Software protocol sharing. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 4. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. StackMax Microplate Handling System. SpectraMax ® iD3 and iD5 Multi-Mode . Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The plate was shaken and incubated in the dark for 10 minutes. 1321N1, a human astrocytoma cell line. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Figure 4. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. . 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. . 5577 Web: Email: info@moldev. Catalog excerpts. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). user upgradeable. 7 mm for all shake intensities. . The plate was shaken and incubated in the dark for 10 minutes. 660-665 Eskdale Road. . Introduction. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. to View Figures. The chamber. )wideand65. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. QuickSync. 9000-0756. SpectraMax i3x Multi-Mode Microplate Reader. . Then, cells were washed with PBS three times and 100 μL of Nano-Glo HiBiT Lytic Reagent was added to. Structured Review. 01 0. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 価格、見積もり、お近くの販売店を. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Itemploysa. infoboxeu@moldev. . SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. SpectraMax Microplate Readers. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Multimode Reader Comparison Chart. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. HTRF IP standard curve. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Wait 20 seconds. The SoftMax Pro Software provides integrated instrument control. . Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Use the 3. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Get a front row view of your cells. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ZERO BIAS - scores, article reviews, protocol conditions and more. 6% for the on-board optics (monochromators). 304 signal level saturation. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. For each kit, the reader was able to detect the lowest dilution as positive. 58% for the detection module and 2. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. Background subtraction was not necessary. . . Settings not required by a reader are indicated by ‘---’. 1. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). Find regional support. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. . 1. D 1 1. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Explore a wealth of applications with the future-ready SpectraMax i3x. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Figure 4. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Microplate reader assays requiring infrared (IR) or red-shifted. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Average 98 stars, based on 1 article reviews. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. The SpectraMax i2X is used for cell migration, cell. Expanded dynamic range The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Wokingham, Berkshire RG41 5TS United Kingdom. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. MitoXpress-Xtra Oxygen Consumption Assay - Agilent. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Replicate values were averaged and imported into Design-Expert 8. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Check software compatibility with Windows 10 and locate your software installer. 020 fmol 0. to View Figures. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. BMG LABTECH VANTAstar. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 0´ 104 8. SpectraMax iD3 and iD5 Register or Sign In. 0. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. Figure 1. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Both Microplate Optimization and Read Height Adjustment were. GFP expression imaged on the cytometer. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. Explore a wealth of applications with the future-ready SpectraMax i3x. 0´ 104 1. Register to download your free eBook today. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Controls containing all reaction. 0001 0. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. 312 gain calibration failed. *On the SpectraMax i3x reader only. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. 12911151) 0. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. com. Both readers feature an 8-channel read head for fast reads and. Description 1. This process should take about 3 - 5 minutes. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. Facilities. Reader spectramax microplate id3 molecular devices multi mode boundaries functionality instrumentSpectramax i3x multi-mode microplate reader with minimax imaging Molecular devices spectramax i3x 使用される 販売のために 価格. Molecular Devices Software. 1 Microplate Data Acquisition & Analysis Software for Windows. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. 22 μ m fi ltration. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Modularity does provide some measure of forward vision into upcoming or future experimental needs. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Microplate readers, multi-mode, SpectraMax® i3x. 7 Figures from 1126 Publications. china@moldev. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. • Removing the transport locks from the PMT shutters and drawers. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. HTRF IP standard curve. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. GloMax Discover System. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. *On the SpectraMax i3x reader only. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. 7. SpectraMax i3x Register or Sign In. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Version 7. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. NORTH AMERICA. 3: The cuvette chamber. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). applications, including dual luciferase and ATP assays. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. 305 reference level saturation. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. . Example: [Setup]. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. user upgradeable. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Cell viability는 세포막 Integrity 또는. Danaher Inc spectramax i3x. TaqMan SNP. Bioz Stars score: 98/100, based on 1 PubMed citations. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Results were plotted as ratio of green/red vs. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Pick the best speed, sensitivity, and price for your needs. 303 unable to cal dark current. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Molecular Interactions. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. . Supported Windows Operating System: See release notes. Other technologies such as imaging. The CellTiter-Glo assay from Promega uses luciferase. Our absorbance plate readers feature our PathCheck Sensor. 315 can't find zero order. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. . The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. com. 2. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Prepare your i3/i3x instrument for shipment. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. Italic and boldface type are used for emphasis. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. SpectraMax i3x Multi-Mode Detection Platform. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. GM2000 Register or Sign In. +1 800-635-5577. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Examples: “Press carefully to engage,” “ Do not press down. Price from $9. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. Not compatible with Home Edition. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. . Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. 99 to $1999. Sensitive TRF detection of two simple, mix-and-measure assay workflows. i3x Specifications. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. . SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Citation 2002). 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. detection cartridge LUM96: 59. Calcium.